PCR single strand conformational polymorphism can be used to detect the gene encoding SHV-7 extended-spectrum beta-lactamase and to identify different SHV genes within the same strain
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منابع مشابه
PCR single strand conformational polymorphism can be used to detect the gene encoding SHV-7 extended-spectrum beta-lactamase and to identify different SHV genes within the same strain.
The PCR single strand conformational polymorphism (PCR-SSCP) technique described to identify mutants of the SHV beta-lactamases was extended to identify an SHV-7 type beta-lactamase. This was found in a strain of Klebsiella pneumoniae, the first recorded isolate in the UK to produce this type of enzyme. We also demonstrate that PCR-SSCP can be used to identify more than one SHV beta-lactamase g...
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Background and aim: Antimicrobial resistance is a worldwide problem causing health threats. Escherichia coli is one of the most important bacteria that causes resistance problems. The aim of this study was to evaluate the frequency of Escherichia coli, a extended spectrum beta-lactamase producing CTX-M and SHV, in hospital and non-hospital clinical specimens in Isfahan. Methods: In this stu...
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BACKGROUND & OBJECTIVE Phenotyping is commonly used for detection of extended spectrum beta lactamase (ESBL) production in gram-negative isolates. ESBLs are mainly coded for by three important genes, namely bla(TEM), bla(SHV) and bla(CTX-M). In this study we used a multiplex PCR as a rapid method to identify two common genes (bla(CTX-M) & bla(SHV)) responsible for extended spectrum beta lactama...
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Objective(s) Production of extended-spectrum beta-lactamases (ESBLs) by enteric bacteria continues to be a major problem in hospitals and community. ESBLs producing bacteria cause many serious infections including urinary tract infections, peritonitis, cholangitis and intra-abdominal abscess. The aim of this study was to determine the prevalence of ESBLs producing Escherichia coli and Klebsiel...
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ژورنال
عنوان ژورنال: Journal of Antimicrobial Chemotherapy
سال: 1998
ISSN: 0305-7453,1460-2091
DOI: 10.1093/jac/41.1.123